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Chromatographic Resolution

Introduction

The resolution (Rs) between two peaks in a chromatogram is given by:

where DeltaZ is the separation between peaks A and B; and Wa and Wb are the widths at the base of peaks A and B, respectively.

Acceptable resolution is on the order of Rs = 1.0, and baseline resolution between two peaks (as shown in the figure) requires an Rs > 1.5.


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